Biochemistry 325
| L E C T U R E S Quizzes and Tests will be given during lab time | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Lecture # | Date Text pages 5th ed Text pages 4th ed | Topic(s) | Page | ||||||
| 1 | January 10 (in lab) | Introduction to the course Nobel Laureates in Biochemistry Research interests of the instructor | 1 2 3 | ||||||
| 2 | January 11 | Control [coarse] mechanisms - the Lac-Operon | 5 | ||||||
| 3 | January 16 131-139 | General enzymology; active sites; molecular ecology | 10 | ||||||
| 4 | January 18 139-155 | Kinetics I: Michaelis-Menton Theory | 15 | ||||||
| 5 | January 23 156-183 | Kinetics II: Fine control mechanisms | 20 | ||||||
| 6 | January 25 463- |
| 25 26 | ||||||
| 7 | January 30 486 | Glycolysis B: The EMP continued | 30 | ||||||
| 7 | February 01 486 | The Entner-Douderoff Pathway | 33 | ||||||
| 8 | February 06 515-518 | The Korkes-Gunsälus Intermediate Pathway | 35 | ||||||
| 9 | February 08 | Glycolytic Inhibitors; Poisson Distribution | 40 | ||||||
| 10 | February 13 540-567 | Electron Transport and Oxidative Phosphorylation | 45 | ||||||
| 11 | February 15 | Membranes and Transport (goes with the LPS SDS-PAGE lab) | 50 | ||||||
| 12 | February 20 511-539 | Krebs Cycle; P/O; inhibitors, etc. | 55 | ||||||
| 13 | February 22 | Energetics of the above pathways | 60 | ||||||
| 14 | February 27 504-510 | Pentose Shunt [aka: Hexose monophosphate shunt, HMS] | 65 | ||||||
| 15 | March 01 434-447 |
| 70 75 76 | ||||||
| 16 | March 06 448-462;487-494 | Carbohydrates II: Polymers The Glycogen Cycle Hormal Control of the Glycogen Cycle | 80 | ||||||
| 17 | March 20 | Proteins IIIA: isolation and purification procedures | 85 | ||||||
| 18 | March 22 80-112 | Proteins IV: structural analysis in vitro Synthesis of the Peptide Bond | 90 91 | ||||||
| 19 | March 27 58-72 | Proteins I: amino acids, chemical tests, structural classification | 95 | ||||||
| 20 | March 29 122-130 | Proteins II: degradation (chemical and enzymatic) Practice Problem | 100 101 | ||||||
| 21 | April 03 629-648 | The Nitrogen Pool (Ammonium fixation & Transamination) | 105 | ||||||
| Here a placement quiz will be given to ascertain which of the following may be skipped due the students' having had genetics. | |||||||||
| 22 | April 03 | In vivo Protein Synthesis | 110 | ||||||
| 23 | April 05 568-586 | Fat and Terpene Syntheses | 115 | ||||||
| 24 | April 10 586-603 | LPS electrophoresis | 120 | ||||||
| 25 | April 12 648-656 | Other Lipids | 125 | ||||||
| 26 | April 17 | LPS electrophoresis (gel making) | 130 | ||||||
| 27 | April 19 | LPS electrophoresis (gel development) | 135 | ||||||
| 28 | April 24 | Radio-Isotopes in Biochemistry | 140 | ||||||
| 29 | April 26 | Immunology in vivo | 145 | ||||||
| 30 | May 01 | Carbon-carbon bond formation review | 150 | ||||||
| 31 | May 02 | Vitamin Survey and help session for final exam | 155 | ||||||
| 32 | May 04 1 p.m. | final exam | |||||||
| L A B O R A T O R Y | |||
|---|---|---|---|
| Labs will usually begin with a quiz on previous and current work. There is no published lab manual; handouts will be provided on-line. | |||
| # | Date | Subject | Page |
| 1 | Jan 10 | Intro to course Preparation of source material - 1 Mission Lac-Operon Basic Bacteriological Preparations | 400 401 402 |
| 2 | Jan 17 | Preparation of source material - 2 Looking for catalase and lactase in bacteria and selected eukaryotes Learning how to use substrate analogs and the spectrophotometer In vitro lactase inhibition, In vivo lactase inhibition | 410 411 412 413 |
| 3 | Jan 24 | Qualitative and Quantitative Methods in Protein Chemistry | 420 |
| 4 | Jan 31 | Paper chromatography of amino acids and their derivatives amino acids, proteins and enymatic activities with unknowns! RF values of amino acids RF values of DNP-amino acid derivatives | 430 436 437 |
| 5 | Feb 07 | Qualitative and Quantitative Methods in Carbohydrate Chemistry using chromatographic and other techniques such as optical rotation and chemical tests | 440 |
| 6 | Feb 14 | A Study of the Effects of Fever on E. coli Lipopolysaccharide Set up and preparation | 450 |
| 7 | Feb 21 | SDS-PAGE and Development of E. coli LPS (detergent augmented polyacrylamide gel electrophoresis and silver staining of gels) | 460 |
| 8 | Feb 28 | Intro quiz to Photosynthesis Photosynthesis Lab A Study of Light Extension #1 Extension #2 | 470 471 472 473 |
| 9 | Mar 07 | Metabolism Optimization of Growth | 480 |
| 10 | Mar21 | Glycolytic Inibitors: Glycerol Fermentation using Sodium Bisulfite | 490 |
| 11 | Mar 28 | Saponification of Olive Oil | 500 |
| 12 | Apr 04 | Isolating the products and byproducts of the previous two labs | 510 |
| 13 | Apr 11 | LPS-electrophoresis via SDS-PAGE | 520 |
| 14 | Apr 18 | Photosynthesis and Pollutants | 530 |
| 15 | Apr 25 | Isolation of DNA and RNA Investigation of some of their chemical and physical properties. (More sophisticated exercises are done in Genetics and will not be done here.) | 540 |
| 16 | May 02 | Help Session for Final Exam (See the last lecture, above) | |
| FINAL EXAM STUFF | |
|---|---|
| I T E M | Page |
| My sketches of the structures of GLUCOSE, ATP, NAD+/NADH | 800 |
| My list of Carbon-Carbon bond formations and breakings | 805 |
| My list of the vitamins covered in this course and their molecular points of action | 810 |
| My list of techniques I've learned in this course. | 815 |