Note the questions do NOT have all the same weight. Min. pts offered = 141; Max pts offered = 195
NEW! You may select up to any THREE questions for doubling their points by circling their numbers.
You may not circle a question more than once (and thus are prevented from getting 4X or 8X!).
| FINAL EXAM - MICROBIOLOGY 150 (Summer 2008) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 4 pts | Purpose?sterilization / quantification of turbidity Name?autoclave / spectrophotometer | 12 15 pts | a. (3 pts) Gram-positive / Gram-negative b. (6 pts) 8.7x106 cfu/mL = c. (6 pts) fraction dead = (1x107-8.7x106)/1x107 = about 1/3 | |||||||||||||||||||||||||||||||||||||||||||||||||||
| 2 2 pts | Purpose?inoculating loop is for transferring bacteria selecting colonies for transfer | 13 15 pts | You got an extra 10 pts for drawing a proper graph.a. (9 pts) about 3 hrs: 1:30 to 4:30 b. (6 pts) doubling time = 25 minutes | |||||||||||||||||||||||||||||||||||||||||||||||||||
| 3 2 pts | Name? incubator | 14 4 pts | Materials and Methods | |||||||||||||||||||||||||||||||||||||||||||||||||||
| 4 8 pts | 230x100=23,000 cfu/mL | 15 8 pts | Write your answer at the approriate place at the bottom. 5 | 2 pts S-35 (from a Mary Lou quiz!) | 16 | 4 pts "g" is the generation time (doubling time)
| 6 | 5 pts TRUE FALSE (R-W) | 17 | 6 pts a b c d e per f g h i j
| 7 | 5 pts TRUE FALSE (R-W) | 18 | 9 pts a. white blood cells | b. complement c. interferon / fever / skin .... but NOT antibodies, which are specific immunity 8 | 8 pts 4X 10X 40X 80X | 19 | 4 pts
9 | 10 pts Write your answer at the approriate | place at the bottom. 20 | 7 pts a. increase stay-the-same decrease | b. antibodies antigens 10 | 8 pts method: On freshly planted lawns of E. coli and Staph. epidermidis, place some grains of pepper. If zones of exclusion (halos) form around the pepper granules, then pepper is antibacterial. | 21 | 10 pts Write your answer at the approriate | place at the bottom. 11 | 5 pts
22 | 0 pts (bonus!) Name:
| 9 | Plate on MacConkey agar (milk bacteria, which are G(+), won't grow. Incubate above 40c. Select red colonies of E. coli.
| 15 | If you got lots of bacteria but of only one or two types, then these must have been grown up elsewhere (inside Mommy), but if you got a few bacteria in only those few seconds (post partum) of many different types, these would have come from the environment.
| 21 | Most people did not read the question! It asked about the benefits of dairy bacteria to the MOTHER - not the infant. They are "guard dogs" against mastitis.
| | |||||||||||||||||||