POST-CLASS QUESTIONS for Week #2
(individual; open book; turn in June 10, 2008)

NAME

Grades will be curved providing you correctly answer at least 4 of these:

1. Bacteria differ from viruses in that
(a) bacteria have ribosomes, but viruses do not
(b) bacteria contain both DNA and RNA, while viruses have only one but not both
(c) axenic cultures of bacteria can be cultured, but viruses cannot grow independently
(d) all of the above are true
(e) only a and b are true 8. A bacterium is an example of a
(a) prokaryotic cell
(b) eukaryotic cell
(c) plant cell
(d) all of the above
2. All cells - including those of bacteria - contain
(a) a nucleus, plasma membrane, and cytoplasm
(b) a cell wall, nucleus, and cytoplasm
(c) DNA, plasma membrane, and cytoplasm
(d) mitochondria, plasma membrane, and cytoplasm 9. Methylene blue
(a) is used to kill cells that are moving too quickly to observe
(b) renders cells nontoxic
(c) is a portion of the electromagnetic spectrum used by green plant cells
(d) is a biological stain used to increase contrast of cellular constituents
(e) none of the above
3. Prokaryotic cells lack
(a) DNA
(b) a true nucleus
(c) a cell wall
(d) all of the above
(e) none of the above 10. Components typical of both plant and bacterial cells but not of animal cells are (circle all correct answers):
(a) nuclei
(b) cell walls
(c) mitochondria
(d) ribosomes
(e) none of the above
4. Bacterial endotoxin refers to
(a) flagella
(b) the cell wall
(c) lipopolysaccharide
(d) a gene in the nucleus
(e) both b and c 11. The terms "strepto-" and "staphylo-" refer to
(a) certain Gram-negative bacteria
(b) certain Gram-positive bacteria
(c) certain spherically shaped bacteria
(d) is none of the above
(e) only b and c.
5. A "cfu"
(a) grows on an agar surface
(b) may be seen with a microscope
(c) possesses a plasma membrane
(d) possesses smaller ribosomes than do eukaryotes
(e) all of the above
(f) only two of the above, but not e 12. Bacterial DNA comes in which of the following varieties?
(a) circular genome, plasmids, and ribosomes
(b) circular genome and mesosomes
(c) circular genome and ribosomes
(d) circular genome, plasmids and episomes
(e) none of the above.
6. Cheryl made a 10-fold serial dilution and plated 10μL samples on a plate she labeled "C-3." The next day she obtained these counts in her four lanes:
TMTC, 92, 12, 1.
(a) the plate should be disgarded
(b) there were 9.2x10⁷ cfu/mL in the original
(c) there were 9.2x10⁶ cfu/mL in the original
(d) there were 1.2x10⁷ cfu/mL in the original 13. Diameters of zones of inhibition (halos) around antibacterial disks is dependent upon
(a) the relative susceptibility of the target organism
(b) the concentration of the inhibitory agent
(c) the molecular weight of the inhibitory agent
(d) only two of a, b and c
(e) all three of a, b and c.
7. Mary Lou made a 10-fold serial dilution and plated 10μL samples on a plate she labeled "ML-3." The next day she obtained these counts in her four lanes:
113, 82, 52, 19.
(a) the plate should be disgarded
(b) there were 8.2x10⁷ cfu/mL in the original
(c) there were 8.2x10⁶ cfu/mL in the original
(d) there were 1.9x10⁹ cfu/mL in the original 14. If a bacterium can only grow on a medium containing many different amino acids, and "vitamins" (trace organic compounds)
(a) it must grow very slowly
(b) its genome must be very large
(c) it is said to possess a "simple" nutrition
(d) it will stain with methylene blue
(e) none of the above

	1. Bacteria differ from viruses in that (a) bacteria have ribosomes, but viruses do not (b) bacteria contain both DNA and RNA, while viruses have only one but not both (c) axenic cultures of bacteria can be cultured, but viruses cannot grow independently (d) all of the above are true (e) only a and b are true			8. A bacterium is an example of a (a) prokaryotic cell (b) eukaryotic cell (c) plant cell (d) all of the above
	2. All cells - including those of bacteria - contain (a) a nucleus, plasma membrane, and cytoplasm (b) a cell wall, nucleus, and cytoplasm (c) DNA, plasma membrane, and cytoplasm (d) mitochondria, plasma membrane, and cytoplasm			9. Methylene blue (a) is used to kill cells that are moving too quickly to observe (b) renders cells nontoxic (c) is a portion of the electromagnetic spectrum used by green plant cells (d) is a biological stain used to increase contrast of cellular constituents (e) none of the above
	3. Prokaryotic cells lack (a) DNA (b) a true nucleus (c) a cell wall (d) all of the above (e) none of the above			10. Components typical of both plant and bacterial cells but not of animal cells are (circle all correct answers): (a) nuclei (b) cell walls (c) mitochondria (d) ribosomes (e) none of the above
	4. Bacterial endotoxin refers to (a) flagella (b) the cell wall (c) lipopolysaccharide (d) a gene in the nucleus (e) both b and c			11. The terms "strepto-" and "staphylo-" refer to (a) certain Gram-negative bacteria (b) certain Gram-positive bacteria (c) certain spherically shaped bacteria (d) is none of the above (e) only b and c.
	5. A "cfu" (a) grows on an agar surface (b) may be seen with a microscope (c) possesses a plasma membrane (d) possesses smaller ribosomes than do eukaryotes (e) all of the above (f) only two of the above, but not e			12. Bacterial DNA comes in which of the following varieties? (a) circular genome, plasmids, and ribosomes (b) circular genome and mesosomes (c) circular genome and ribosomes (d) circular genome, plasmids and episomes (e) none of the above.
	6. Cheryl made a 10-fold serial dilution and plated 10μL samples on a plate she labeled "C-3." The next day she obtained these counts in her four lanes: TMTC, 92, 12, 1. (a) the plate should be disgarded (b) there were 9.2x10⁷ cfu/mL in the original (c) there were 9.2x10⁶ cfu/mL in the original (d) there were 1.2x10⁷ cfu/mL in the original			13. Diameters of zones of inhibition (halos) around antibacterial disks is dependent upon (a) the relative susceptibility of the target organism (b) the concentration of the inhibitory agent (c) the molecular weight of the inhibitory agent (d) only two of a, b and c (e) all three of a, b and c.
	7. Mary Lou made a 10-fold serial dilution and plated 10μL samples on a plate she labeled "ML-3." The next day she obtained these counts in her four lanes: 113, 82, 52, 19. (a) the plate should be disgarded (b) there were 8.2x10⁷ cfu/mL in the original (c) there were 8.2x10⁶ cfu/mL in the original (d) there were 1.9x10⁹ cfu/mL in the original			14. If a bacterium can only grow on a medium containing many different amino acids, and "vitamins" (trace organic compounds) (a) it must grow very slowly (b) its genome must be very large (c) it is said to possess a "simple" nutrition (d) it will stain with methylene blue (e) none of the above