PRE-CLASS QUESTIONS for Week #6
(individual; open book; turn in June 24, 2008)
(lose 20% if turned in on June 25; lose everything if turned in later!)

Grades will be curved providing you correctly answer at least 2 of these:
Put your answers on the answer sheet.

1. The most commonly employed reagent for testing for the presence of starch is:
(a) methylene blue
(b) hydrogen peroxide
(c) amylose
(d) tincture of iodine (aqueous KI₃)
(e) there are no recognized tests for starch
4. Gram-positive bacteria canNOT grow on which of the following
(a) nutrient agar
(b) EMB agar
(c) MacConkey agar
(d) tryptic soy agar
(e) b and c only
(e) all of the above

2. A common test for hemolytic activity in bacteria is plating them on nutrient agar containing
(a) Sudan black IV
(b) methyl red
(c) defibrinated sheep's blood
(d) 6.5% NaCl
(e) both a and c
(f) none of the above would work
5. The lactic acid bacteria generally
(a) require many different amino acids
(b) can grow on an aqueous medium consisting only of minerals and glucose in the presence of light
(c) are catalase positive
(d) can grow only in the absence of air
(e) both c and d
(f) none of the above

3. The first step in a common test for proteolytic activity in bacteria is plating them on nutrient agar containing
(a) gelatin
(b) methyl red
(c) defibrinated sheep's blood
(d) 1% ammonium chloride
(e) both a and b
(f) none of the above would work
6. The bacterial growth curve
(a) is used to calculate the time it takes for the population to double
(b) consists of four growth phases: lag, exponential (log), stationary and death
(c) can be derived from plate count data
(d) can be derived from data obtained from machines which measure turbidity
(e) all of the above
(f) both c and d only

	1. The most commonly employed reagent for testing for the presence of starch is: (a) methylene blue (b) hydrogen peroxide (c) amylose (d) tincture of iodine (aqueous KI₃) (e) there are no recognized tests for starch			4. Gram-positive bacteria canNOT grow on which of the following (a) nutrient agar (b) EMB agar (c) MacConkey agar (d) tryptic soy agar (e) b and c only (e) all of the above
	2. A common test for hemolytic activity in bacteria is plating them on nutrient agar containing (a) Sudan black IV (b) methyl red (c) defibrinated sheep's blood (d) 6.5% NaCl (e) both a and c (f) none of the above would work			5. The lactic acid bacteria generally (a) require many different amino acids (b) can grow on an aqueous medium consisting only of minerals and glucose in the presence of light (c) are catalase positive (d) can grow only in the absence of air (e) both c and d (f) none of the above
	3. The first step in a common test for proteolytic activity in bacteria is plating them on nutrient agar containing (a) gelatin (b) methyl red (c) defibrinated sheep's blood (d) 1% ammonium chloride (e) both a and b (f) none of the above would work			6. The bacterial growth curve (a) is used to calculate the time it takes for the population to double (b) consists of four growth phases: lag, exponential (log), stationary and death (c) can be derived from plate count data (d) can be derived from data obtained from machines which measure turbidity (e) all of the above (f) both c and d only