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Gel Ingredients

Item BOTTOM GEL TOP GEL
10% 12% 15%
A/Bis
(29.2%/0.8%) 23.0 ml 28.0 ml 35.0 ml A/Bis
(29.2%/0.8%) 1.3 ml
Tris
1.5M; pH 8.8 17.5 ml 10.0 ml 17.5 ml Tris
0.5M; pH 6.8 0.5 ml
Water 28.7 ml 31.2 ml 16.7 ml Water 8.2 ml
10% SDS 0.7 ml 0.7 ml 0.7 ml 10% SDS 100 μL
TEMED 35 μL 35 μL 35 μL TEMED 6.0 μL
10% APS
Ammonium Per-Sulfate 175 μL 175 μL 175 μL 10% APS 50 μL

Item	BOTTOM GEL			TOP GEL
	10%	12%	15%
A/Bis (29.2%/0.8%)	23.0 ml	28.0 ml	35.0 ml	A/Bis (29.2%/0.8%)	1.3 ml
Tris 1.5M; pH 8.8	17.5 ml	10.0 ml	17.5 ml	Tris 0.5M; pH 6.8	0.5 ml
Water	28.7 ml	31.2 ml	16.7 ml	Water	8.2 ml
10% SDS	0.7 ml	0.7 ml	0.7 ml	10% SDS	100 μL
TEMED	35 μL	35 μL	35 μL	TEMED	6.0 μL
10% APS Ammonium Per-Sulfate	175 μL	175 μL	175 μL	10% APS	50 μL