Phage Lambda: The Molecular Biologist's Other Main Tool

1. THE ANATOMY OF LAMBDA
   1. Morphology
   2. The Genetic Material
      1. dsDNA
      2. 30.8 kdal = 46.5 kbp.
      3. sticky ends of 12 bases ("cos" region)
      4. Its genetic map
   3. Host range - see previous lecture on T4 rII mutants

2. A DEPENDENT VIRUS
   1. How to test for dependency
      

   2. What does lambda need?

3. A TEMPERATE VIRUS <<<----- This is one of the most important topics in the course !!!
   1. The lytic cycle
   2. Lysogeny
      1. Nomenclature
      2. Simple Observations of lysogeny
         • Insertion into the host chromosome
           

         • Methods of induction
           1. ultraviolet
           2. thermal and nutrient
           3. zygotic
              1. What is sexduction in E. coli?
              2. Evidence for zygotic induction.
                 

         • How lysogeny works
           1. How is the prophage carried from generation to generation?
           2. How induction generally works
              (To facilitate your studying, print out BOTH the following figure as well as the schedule of events in the decision to go lytic or to lysogenize.)
           

         • The genetic clockworks of lysogeny/lytic <<<---Really hot stuff!

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