Other DNA Coliphages

Lecture 16: Other DNA Coliphages


  1. T5

    1. dsDNA = 8x107 dal (T4 dsDNA = 1.2x108 dal.)
    2. Central 10% of genome expendible
    3. Two-stage insertion of DNA into host: 8% first, then the remainder
    4. About 9% terminal redundancy

    5. dsDNA = 2.4x107 dal.
    6. Highly linear map; distinct, constant termini
    7. Polycistronic mRNA's need host processing to short mRNA's
      This was one of the first proofs that post-transcriptional processing was not unique to eukaryotes!
    8. Only a few promoters and one stop signal.
    9. DNA packaging is not by headfuls (as T4)
    10. Virion head contains core protein (DNA packaging?)

  2. T7 Relatives

    1. T3, a very close relative

      1. Large sections of the DNA homologous to T7 DNA
      2. The action of SAMase to augment its invasion of host

    2. ø2, only a few stretches of DNA homology with T7
      Might this be a subject for the study of evolution in phages?

  3. T1? What happened to its discussion? (No one wants to work with it because it is dessicant resistant and blows around in the dust. Whenever it settles into E. coli cultures, the bacteria soon lyse! I.e.: T1 is too contagious for most labs. Hence, little is known about it.)

  4. ssDNA coliphages - both are male (F+) specific

    1. f1 - a cylindrical phage that afixes to the end of the F-pilus. Ouch!
    2. øX174 (an old friend)


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