Proof of the Existence of Messenger RNA

Five Points of Genetic Expression - II
Proof of the Existence of Messenger RNA
Classday 7

  1. The Implications of an "RNA" Virus (1956) (Gierer & Schramm)

    1. Can RNA be genetic material?

  2. Discovery of Short-Lived RNA (1956) (Volkin & Astrachan)

    1. Pulse labelling in principle

    2. Pulse-Chased labelled E. coli

    3. Pulse-Chased labelled infected cells

  3. Transcription Hypothesized (1961) (Jacob & Monod)

  4. Complementarity of RNA and DNA

    1. DNA/RNA hybridization

      1. In liquid (using isopycnography)

      2. Nitrocellulose

        1. Columns

          1. Isolation of rII DNA

          2. Isolation of rII m-RNA

        2. Filters

        3. Kinetics of hybridization

        4. Pulse-labelled cells and THE SINGLE BURST EXPERIMENT

  5. Ramifications and future findings

    1. Relatedness of species

      1. DNA-DNA hybridization and Homo vs apes; E.coli vs Salmonella; etc.

      2. Interspecific RNA-DNA hybridizations

    2. Tandem alleles

    3. Genetic clocks

    4. Structure of the Host Chromosome - The Replicon

    5. etc.

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