(Point D of the Five Points of Genetic Expression)
Protein Synthesis
Classday 9

1. Nirenberg's Experience in Moscow ("He's not a member of the club.")

2. The components of the natural (in vivo) protein synthesis system.
   1. Ribosomes
   2. tRNA
   3. ATP, GTP
   4. Amino acids
   5. Cytoplasmic fluid (enzymes)
   6. mRNA

3. The in vitro system
   1. Source of components A through E
   2. Source of the mRNA

4. Implications
   1. Use in the determination of the genetic code
      1. Use of natural mRNA's
      2. Use of artificial mRNA's

   2. Use in determining what various genes produce
      1. Software
         1. Punctuation (Sinshimer's "Story of Life")
         2. Punctuation factors
            1. Initiation
            2. Elongation
            3. Termination

      2. Damaged Software
         1. Missense
         2. Nonsense
         3. Suppressors

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